Estimation and Comparison of Cardioprotective Potential of Drakshasava Prepared by Traditional and Modern Methods on Isoproterenol Induced Myocardial Infarction in Albino Rats

Abstract

This study was designed to estimate the cardio-protective potential of Drakshasava-T and Drakshasava-M which was prepared by traditional and modern methods respectively, and also of Dabur Drakshasava on the basis of histopathological and biochemical parameters in the Isoproterenol (ISO) induced Myocardial infarction (MI) in albino rats and to compare with Inderal*10 (contains Propranolol hydrochloride 10 mg), which is a known cardioprotective agent. Wistar albino rats of either sex weighing between 200–220g were divided into six groups as – normal, ISO control, Inderal*10 treated and Drakshasava-T, Drakshasava-M and Dabur Drakshasava treatment groups. Drakshasava-T, Drakshasava-M and Dabur Drakshasava were administered at the dose of 2 ml/kg body weight and Inderal*10 at the dose of 10mg/kg orally for 30 days. On 29th and 30th day, the rats in the ISO control and in all the treatment groups were given ISO (85mg/kg, i.p.) at an interval of 24 hour. On 31st day, blood was collected by retro-orbital bleeding under mild ether anaesthesia and level of serum marker enzymes viz. Creatine Kinase (CK-MB), Lactate Dehydrogenase (LDH), Aspartate amino- transferase (AST) and Alanine amino-transferase (ALT) were determined and serum lipid profile was also measured. After that, these animals were subsequently sacrificed with a higher dose of ether anaesthesia, hearts were removed, weighed and immediately processed for histopathological and biochemical studies. Relative heart weight to body weight ratio was also studied. A significant decrease (P<0.001) in Glutathione (GSH) and increase (P<0.001) in lipid per-oxidation marker Malonyldialdehyde (MDA) level was observed in the hearts of ISO Control group as compared to the normal group which was significantly (P<0.001) prevented by all these test formulations as Drakshasava -T, M and Dabur Drakshasava. Increased level of serum marker enzymes as CK-MB, LDH, AST and ALT as well as lipid profile in ISO induced myocardial infarction in rats was restored with the pretreatment of all these test formulations. Furthermore, the increased heart weight, relative heart weight to body weight ratio and cardiac architecture damage were also found improved with the pretreatment of all these test formulations. Thus, all these test formulations were found nearby equally effective to that of standard cardio-protective agent Inderal *10. Experimental finding suggests that cardio-protective activity of Drakshasava-T, DrakshasavaM and Dabur Drakshasava may be due to an augmentation of endogenous antioxidants as GSH and inhibition of lipid per-oxidation of cardiac membrane. The presence of enriched phenolic and flavonoid compounds in all these test formulations act as antioxidants and might be helpful in stabilizing the cardiac membrane.  Keywords: Cardioprotectives, Myocardial Infarction, Isoproterenol, Drakshasava-T, Drakshasava-M

Subhajit Ghosh, P.N.Murthy, H.Joshi. Estimation and Comparison of Cardioprotective Potential of Drakshasava Prepared by Traditional and Modern Methods on Isoproterenol Induced Myocardial Infarction in Albino Rats. Research and Reviews: A Journal of Ayurvedic Science, Yoga and Naturopathy. 2017; 4(2): 27–37p.