Formulation Development of Otic In Situ Gel containing Lomefloxacin Hydrochloride

Abstract

Abstract

The present study was aimed at formulation development of a sustained release otic in situ gel of lomefloxacin hydrochloride for the treatment of chronic suppurative otitis media. Poloxamer 407 was used as the thermoreversible gelling agent. Poloxamer concentration in the range of 15–20% w/v was tried. Poloxamer 407 at 16% w/v gave satisfactory in situ gel forming formulations as it gelled near physiological temperature. Poloxamer 407 with various viscosity enhancing agents like methocel K100M, methocel K4M, carbopol 974P were tried in the range of 0.1–0.3% w/v. The formulations were evaluated for appearance/clarity, gelation temperature and gelling time, pH, rheological characteristics, drug content, in vitro drug release studies, ex vivo studies, release kinetics and stability studies. FT-IR spectroscopy was used to know drug and polymer incompatibilities. The viscosity of the formulations increased with increase in concentration of viscosity enhancers. In vitro release of selected formulations through synthetic membrane showed sustained release of drug over a period of 9 h. Ex vivo diffusion study for optimized formulation was also carried out through biological membrane. In case of pure drug, the release was more than 90% in 4 h and from in situ gel; the release was 55.38% at 9 h. Thus it can be a promising drug delivery system with sustained release and reduce frequency of administration providing better patient compliance.

Keywords: Lomefloxacin hydrochloride, in situ gel, poloxamer 407, gelation temperature, viscosity enhancing agents

Cite this Article

Niyas A M, Narayana Charyulu R, Jobin Jose. Formulation Development of Otic In Situ Gel containing Lomefloxacin Hydrochloride. Research & Reviews: A Journal of Drug Formulation, Development and Production. 2016; 3(1): 20–29p.