A Novel Technique for Stability Testing of Alliin and Sennosides in Unani Formulations

Abstract

Unani system of medicines is a science which deals with the preventive and promotive aspects of health of human beings and health problems that arise due to factors like ecological and/or environmental, which include humours categorized as blood, phlegm, yellow bile and black bile, and different biological fluids circulating in various vessels of the body. There is significant mandate in drug testing for a specific and precise analytical method for the identification of Alliin and Sennosides in various Unani formulations. A novel technique which combines high performance liquid chromatography and mass spectrometry (LC-MS/MS) will provide unequivocal thumbprint information for estimation of Alliin and Sennosides in Unani formulations. The objective of the current study is to develop an efficient, simple, cost-effective and steady high-performance liquid chromatography method for the quantification of Alliin and Sennosides in Unani formulations and helps in stability testing of these compounds. The separation of the compounds was achieved in C18 column and positive (Alliin)/negative (Sennosides) ion mode was used for detection in ESI-MS detection.

Keywords: Alliin, imipramine, sennosides, hydrochlorothiazide, LC-MS/MS