Molecular Methods to Improve the Insecticidal Action of Bacillus thuringiensis in Biotechnology Arena
Abstract
Abstract
Bacillus thuringiensis (or Bt) is a Gram-positive, soil-dwelling bacterium, frequently used as an organic pesticide. B. thuringiensis also arises naturally in the gut of caterpillars of several kinds of moths and butterflies, as well on foliage surfaces, marine environments, animal feces, insect-rich surroundings, and flour mills and grain-storage facilities. All through sporulation, various Bt strains harvest crystal proteins (proteinaceous inclusions), termed δ-endotoxins, that have insecticidal action. This has led to their use as pesticides, and more recently to genetically improved crops using Bt genes, such as Bt corn. Numerous crystal-producing Bt strains, however, do not have insecticidal properties. We define natural and in vitro growth of Cry toxins, as well as their significance in the mechanism of action for insect control. Furthermore, the use of DNA shuffling to develop two Bt toxins will be conversed together with in silico analyses of the generated mutations to evaluate their possible effect on protein structure and cytotoxicity.
Keywords: Bacillus thuringiensis, Cry toxins, genes, pest
Cite this Article
Vishakha Singhal. Molecular Methods to Improve the Insecticidal Action of Bacillus thuringiensis in Biotechnology Arena. Research & Reviews: A Journal of Bioinformatics. 2017; 4(1): 31–35p.
Downloads
Published
Issue
Section
License
Copyright Transfer and Declaration Form (Please compile this form, sign and send by e-mail and post)
Journal Title:
Title of the Paper:
Corresponding Author’s Information:
Name:
Address:
E-mail:
Contact Number:
It is herein agreed that:
The copyright to the above-listed unpublished and original article is transferred to STM Journals. This copyright transfer covers the exclusive right to reproduce and distribute the contribution, including reprints, translations, photographic reproductions, microform, electronic form (offline, online), or any other reproductions of similar nature. I/We declare that above manuscript is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere. I/ We warrant(s) that his/her/their contribution is original, except for such excerpts from copyrighted works as may be included with the permission of the copyright holder and author thereof, that it contains no libelous statements, and does not infringe on any copyright, trademark, patent, statutory right, or propriety right of others. I/We will not publish his/her/their above said contribution anywhere else without the prior written permission of the publisher unless it has been changed substantially.
I/We also agree to the authorship of the article in the following order:
Author(s) Name Signature(s)
1. ________________
2. ________________
3. ________________
4. ________________
The author(s) agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal (comments if any to the editor can be added below).
