Phylogenomics Analysis of Fibrillar Collagen Gene Family

Abstract

Fibrillar collagens are extracellular matrix proteins conserved in complex multicellular animals and are involved in teeth and bone formation. So far, 28 types of collagen have been identified among them, the most common types are collagen I, II, III, IV and V. Mutations results in a number of severe diseases including osteogenesis imperfecta, aortic aneurysms, Alport’s syndrome and even some forms of cancer. In the current study, protein data for a wide variety of vertebrate and invertebrate genomes were taken to understand the phylogeny picture of highly significant and functionally diverse fibrillar collagen family. Phylogenetic tree reveals that fibrillar collagen gene originated at the root of vertebrates and invertebrates lineage. COL2A1 and COL1A1 have evolved at faster rate as a result of the most recent duplication, while COL5A2 has diverged first as the result of the most ancient duplication. Phylogenetic analysis suggests that within the vertebrate lineage, fibrillar collagen gene got highly diversified resulted into its five members, due to selection and functional constraints by the series of four independent duplications. First duplication event diverge COL5A2 from the rest of the members of Fibrillar Collagen Gene Family, whereas in the result of second duplication, COL3A1 get separated from COL1A1/1A2/2A1. Third duplication event leads to the severance of COL1A2 from COL2A1/1A1 and the fourth duplication separates COL2A1 from COL1A1. The topology of the fibrillar collagen gene family member shows that COL5A2 diverged firstly and the remaining family members exhibits the topology of the form (A) (BCD) that favored not 2R hypothesis. This study will assist evolutionary biologist to infer the evolutionary pattern, evolutionary rate and phylogenetic relationships between members of Fibrillar Collagen Gene Family in terms of orthology and paralogy.

Keywords: Fibrillar collagen family, Alport’s syndrome, independent duplications

Cite this Article

Md. Khokhar W. Phylogenomics Analysis of Fibrillar Collagen Gene Family. Research and Reviews: Journal of Computational Biology. 2016; 5(1): 33–38p.