In-silico Phylogenomic Analysis of N-deacetylase/N-sulfotransferase Gene Family Members

Abstract

The macro evolutionary events lead to morphological complexity achieved by higher vertebrates. The extensive gene duplications results in widespread existence of gene families in modern vertebrates.N-deacetylase/sulfotransferase (NDST) gene family are essential for N-deacetylation and N-sulfation of glucosamine (GlcNAc). NDST family is involved in embryonic neurocranium, viscerocranium morphogenesis. Four members of NDST gene family (NDST1, NDST2, NDST3 and NDST4). NDST genes encode a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The phylogenomic analysis of NDST gene family was carried out to check the evolutionary history and functional significance. The colossal amount of diversified protein dataset for a wide variety of vertebrates, Invertebrates was used to derive mode of evolution and conservation of functionally significant diverse gene family residing on human chromosomes. Phylogeny advocates the expansion of multigene family which occurred after diversification of vertebrates from urochordates. Phylogenetic tree reflects chromosomal segmental duplications or small scale duplications which occurred at different time points during chordate evolution. The study will provide better understanding of ancestral relationship and functional behavior of human multigene families for scientist belongs to diversified fields of biology especially comparative and evolutionary genomics biologist.

Keywords: NDST, Phylogenomic analysis, segmental duplication, chromosomes, gene family

Cite this Article

Muhammad Waqas Khokhar, Sonia Kanwal. In-silico Phylogenomic Analysis of N-deacetylase/N-sulfotransferase Gene Family Members. Research & Reviews: Journal of Computational Biology. 2016; 5(2):    7–12p.