Evaluation of Antioxidant Activity of Coumarin Isolated from Eupatorium triplinerve

Abstract

Eupatorium triplinerve (Asteraceae) is a slender herb with narrow lanceolate leaves. Extract of the plant is used as antiseptic, and in the treatment of various ulcers and haemorrhages. Ayapana is a rich source of naturally occurring coumarin chemicals. Coumarins are chemical compounds found in many plants and which usually have a sweet scent—much like newly-mown hay. Coumarin has clinical value as the precursor for several anticoagulant drugs; most notably, one widely prescribed drug called warfarin. Coumarin and methanolic extract were evaluated for various antioxidant assays like DPPH, Superoxide anion radical, nitric oxide, TBARS taking Rutin as standard. Methanolic extract of Eupatorium triplinerve showed better activity against DPPH, Nitric oxide, ABTS, but failed against superoxide and hydrogen peroxide assay. Coumarin showed better activity against DPPH, hydrogen peroxide, ABTS assay but failed against superoxide, Nitric oxide assay. These results clearly indicate that Coumarin is a paramount target for further research in curing various diseases.

Keywords: Eupatorium triplinerve, coumarin, anti-oxidant