UV Spectrophotometric Method for the Estimation of Etoposide and its Application in Study of Polymeric Nanoparticles

Abstract

ABSTRACT

Abstract

A rapid, economical, accurate and simple UV spectrophotometric method was developed for quantification of etoposide in bulk and pharmaceutical formulation and its application in the study of polymeric nanoparticles. The samples were quantified at 284 nm in both methanol and phosphate buffer saline (PBS) at pH 7.4. The method was linear (r2 > 0.99) over the range of 20–100 μg/ml, precise (RSD < 5%), accurate (recovery values close to 100%), selective regarding excipients of the sample, and had a quantitation limit equal to 1.68 μg/ml. The validated method can be successfully employed for routine quality control analyses of polymeric nanoparticles.

Keywords: etoposide, nanoparticles, drug content, drug release, UV spectrophotometry

Cite this Article

Ayyappan T, Shanmugam S, Vetrichelvan T. UV Spectrophotometric Method for the Estimation of Etoposide and its Application in Study of Polymeric Nanoparticles. Trends in Drug Delivery. 2016; 3(1): 46–51p.